Characterization of metabolic changes associated with the functional development of 3D engineered tissues by non-invasive, dynamic measurement of individual cell redox ratios.

نویسندگان

  • Kyle P Quinn
  • Evangelia Bellas
  • Nikolaos Fourligas
  • Kyongbum Lee
  • David L Kaplan
  • Irene Georgakoudi
چکیده

Non-invasive approaches to assess tissue function could improve significantly current methods to diagnose diseases and optimize engineered tissues. In this study, we describe a two-photon excited fluorescence microscopy approach that relies entirely on endogenous fluorophores to dynamically quantify functional metabolic readouts from individual cells within three-dimensional engineered tissues undergoing adipogenic differentiation over six months. Specifically, we employ an automated approach to analyze 3D image volumes and extract a redox ratio of metabolic cofactors. We identify a decrease in redox ratio over the first two months of culture that is associated with stem cell differentiation and lipogenesis. In addition, we demonstrate that the presence of endothelial cells facilitate greater cell numbers deeper within the engineered tissues. Since traditional assessments of engineered tissue structure and function are destructive and logistically intensive, this non-destructive, label-free approach offers a potentially powerful high-content characterization tool for optimizing tissue engineering protocols and assessing engineered tissue implants.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Quantitative metabolic imaging using endogenous fluorescence to detect stem cell differentiation

The non-invasive high-resolution spatial mapping of cell metabolism within tissues could provide substantial advancements in assessing the efficacy of stem cell therapy and understanding tissue development. Here, using two-photon excited fluorescence microscopy, we elucidate the relationships among endogenous cell fluorescence, cell redox state, and the differentiation of human mesenchymal stem...

متن کامل

Bioprinting in Vascularization Strategies

Three-dimensional (3D) printing technology has revolutionized tissue engineering field because of its excellent potential of accurately positioning cell-laden constructs. One of the main challenges in the formation of functional engineered tissues is the lack of an efficient and extensive network of microvessels to support cell viability. By printing vascular cells and appropriate biomaterials,...

متن کامل

Presentation of a Non-invasive Method of Estimating Arterial Stiffness by Modeling Blood Flow and Arterial Wall Based on the Determination of Elastic Module of Arterial Wall

Introduction: Arterial stiffness is an important predictor of cardiovascular risk. Several indices have been  introduced to estimate the arterial stiffness based on the changes in the brachial blood pressure. Since the  substitution  of  the  blood  pressure  changes  in  the  central  arteries  such  as  carotid  with  the  blood  pressure  changes in the brachial results in error in the blood...

متن کامل

A Review of the Function of Circulating Cell Free miRNAs as Promising Biomarkers in Cancer Diagnosis, Treatment, and Metastasis

Cell Free miRNAs are small, non-coding molecules that can be secreted into the bloodstream in very stable forms. These types of miRNAs, like intracellular miRNAs, participate in the control of many biological processes and are expressed in both natural and pathological conditions. Quantitative and qualitative changes in expression of circulating miRNAs are associated with the onset and progress...

متن کامل

Features and Methods of Making Nanofibers by Electrospinning, Phase Separation and Self-assembly

One of the major challenges in the field of tissue engineering is the production of scaffolding in nano-scale. The study of structural-functional connections in pathological and normal tissues with biologically active alternatives or engineered materials has been developed. Extracellular Matrix (ECM) is a suitable environment consisting of gelatin, elastin and collagen types I, II and III, etc....

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Biomaterials

دوره 33 21  شماره 

صفحات  -

تاریخ انتشار 2012